Archives of Neuroscience

Published by: Kowsar

Effects of 660 nm Low Level Laser Therapy on Neuropathic Pain Relief Following Chronic Constriction Injury in Rat Sciatic Nerve

Masoume Masoumipoor 1 , Seyed Behnam Jameie 1 , 2 , 3 , * , Atusa Janzadeh 2 , 4 , Farinaz Nasirinezhad 4 , 5 , Mahdie Kerdari 2 and Maryam Soleimani 3
Authors Information
1 Department of Nuclear Engineering, Islamic Azad University, Tehran, IR Iran
2 Department of Medical Basic Sciences, Iran University of Medical Sciences, Tehran, IR Iran
3 Department of Anatomy, Iran University of Medical Sciences, Tehran, IR Iran
4 Research Center of Physiology, Iran University of Medical Sciences, Tehran, IR Iran
5 Department of Physiology, Iran University of Medical Sciences, Tehran, IR Iran
Article information
  • Archives of Neuroscience: July 01, 2014, 1 (2); 76-81
  • Published Online: July 1, 2014
  • Article Type: Research Article
  • Received: July 22, 2013
  • Accepted: September 9, 2013
  • DOI: 10.5812/archneurosci.13740

To Cite: Masoumipoor M, Jameie S B, Janzadeh A, Nasirinezhad F, Kerdari M, et al. Effects of 660 nm Low Level Laser Therapy on Neuropathic Pain Relief Following Chronic Constriction Injury in Rat Sciatic Nerve, Arch Neurosci. 2014 ; 1(2):76-81. doi: 10.5812/archneurosci.13740.

Copyright © 2014, Tehran University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Merskey H, Bogduk N. Classification of chronic pain: descriptions of chronic pain syndromes and definitions of pain terms. 1994; : 209-14
  • 2. Wang LX, Wang ZJ. Animal and cellular models of chronic pain. Adv Drug Deliver Rev. 2003; 55(8): 949-65[DOI]
  • 3. Woolf CJ, Mannion RJ. Neuropathic pain: aetiology, symptoms, mechanisms, and management. Lancet. 1999; 353(9168): 1959-64[DOI][PubMed]
  • 4. Zimmermann M. Pathobiology of neuropathic pain. Eur J Pharmacol. 2001; 429(1-3): 23-37[DOI]
  • 5. Neuronal plasticity and pain following peripheral tissue inflammation or nerve injury. Proceedings of the VIth World Congress on Pain. : 263-76
  • 6. Woolf CJ. Windup and central sensitization are not equivalent. Pain. 1996; 66(2-3): 105-8[PubMed]
  • 7. Bennett GJ, Xie YK. A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain. 1988; 33(1): 87-107[DOI]
  • 8. Bennett GJ, Chung JM, Seltzer Z. Models of neuropathic pain in the rat. Curr Protoc Pharmacol. 2003; : 5.32. 1-5.32. 16
  • 9. D'amour FE, Smith DL. A method for determining loss of pain sensation. J Pharmacol Exp Ther. 1941; 72(1): 74-9
  • 10. Hargreaves K, Dubner R, Brown F, Flores C, Joris J. A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain. 1988; 32(1): 77-88[PubMed]
  • 11. Espejo EF, Mir D. Structure of the rat's behaviour in the hot plate test. Behav Brain Res. 1993; 56(2): 171-6[DOI]
  • 12. Woolfe G, Macdonald AD. The evaluation of the analgesic action of pethidine hydrochloride (demerol). J Pharmacol Exp. 1944; 80: 300-7
  • 13. Chaplan SR, Bach FW, Pogrel JW, Chung JM, Yaksh TL. Quantitative assessment of tactile allodynia in the rat paw. J Neurosci Methods. 1994; 53(1): 55-63[PubMed]
  • 14. Dubner R. Pain . 1989; : 247-56
  • 15. Mendonca AC, Barbieri CH, Mazzer N. Directly applied low intensity direct electric current enhances peripheral nerve regeneration in rats. J Neurosci Methods. 2003; 129(2): 183-90[PubMed]
  • 16. Huang YY, Chen AC, Carroll JD, Hamblin MR. Biphasic dose response in low level light therapy. Dose Response. 2009; 7(4): 358-83[DOI][PubMed]
  • 17. Ohshiro T, Calderhead RG, Walker JB. Low level laser therapy: a practical introduction. 1988;
  • 18. Schindl A, Schindl M, Schindl L, Jurecka W, Honigsmann H, Breier F. Increased dermal angiogenesis after low-intensity laser therapy for a chronic radiation ulcer determined by a video measuring system. J Am Acad Dermatol. 1999; 40(3): 481-4[PubMed]
  • 19. dos Reis FA, Belchior AC, de Carvalho Pde T, da Silva BA, Pereira DM, Silva IS, et al. Effect of laser therapy (660 nm) on recovery of the sciatic nerve in rats after injury through neurotmesis followed by epineural anastomosis. Lasers Med Sci. 2009; 24(5): 741-7[DOI][PubMed]
  • 20. Belchior AC, dos Reis FA, Nicolau RA, Silva IS, Perreira DM, de Carvalho Pde T. Influence of laser (660 nm) on functional recovery of the sciatic nerve in rats following crushing lesion. Lasers Med Sci. 2009; 24(6): 893-9[DOI][PubMed]
  • 21. Barbosa RI, Marcolino AM, de Jesus Guirro RR, Mazzer N, Barbieri CH, de Cassia Registro Fonseca M. Comparative effects of wavelengths of low-power laser in regeneration of sciatic nerve in rats following crushing lesion. Lasers Med Sci. 2010; 25(3): 423-30[DOI][PubMed]
  • 22. Hsieh YL, Chou LW, Chang PL, Yang CC, Kao MJ, Hong CZ. Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1alpha (HIF-1alpha). J Comp Neurol. 2012; 520(13): 2903-16[DOI][PubMed]
  • 23. Bertolini GR, Artifon EL, Silva TS, Cunha DM, Vigo PR. Low-level laser therapy, at 830 nm, for pain reduction in experimental model of rats with sciatica. Arq Neuropsiquiatr. 2011; 69(2B): 356-9[PubMed]
  • 24. Randall LO, Selitto JJ. A method for measurement of analgesic activity on inflamed tissue. Arch Int Pharmacodyn Ther. 1957; 111(4): 409-19[PubMed]
  • 25. Cui JG, Holmin S, Mathiesen T, Meyerson BA, Linderoth B. Possible role of inflammatory mediators in tactile hypersensitivity in rat models of mononeuropathy. Pain. 2000; 88(3): 239-48[PubMed]
  • 26. Leung L, Cahill CM. TNF-alpha and neuropathic pain--a review. J Neuroinflammation. 2010; 7: 27[DOI][PubMed]
  • 27. Martucci C, Trovato AE, Costa B, Borsani E, Franchi S, Magnaghi V, et al. The purinergic antagonist PPADS reduces pain related behaviours and interleukin-1 beta, interleukin-6, iNOS and nNOS overproduction in central and peripheral nervous system after peripheral neuropathy in mice. Pain. 2008; 137(1): 81-95[DOI][PubMed]
  • 28. Sommer C, Kress M. Recent findings on how proinflammatory cytokines cause pain: peripheral mechanisms in inflammatory and neuropathic hyperalgesia. Neurosci Lett. 2004; 361(1-3): 184-7[DOI][PubMed]
  • 29. Devor M, White DM, Goetzl EJ, Levine JD. Eicosanoids, but not tachykinins, excite C-fiber endings in rat sciatic nerve-end neuromas. Neuroreport. 1992; 3(1): 21-4
  • 30. Higgs EA, Moncada S, Vane JR. Inflammatory effects of prostacyclin (PGI2) and 6-oxo-PGF1alpha in the rat paw. Prostaglandins. 1978; 16(2): 153-62[PubMed]
  • 31. Schepelmann K, Messlinger K, Schaible HG, Schmidt RF. Inflammatory mediators and nociception in the joint: excitation and sensitization of slowly conducting afferent fibers of cat's knee by prostaglandin I2. Neuroscience. 1992; 50(1): 237-47[PubMed]
  • 32. Cuzzocrea S, Thiemermann C, Salvemini D. Potential therapeutic effect of antioxidant therapy in shock and inflammation. Curr Med Chem. 2004; 11(9): 1147-62[PubMed]
  • 33. Torres SH, De Sanctis JB, de L Briceno M, Hernandez N, Finol HJ. Inflammation and nitric oxide production in skeletal muscle of type 2 diabetic patients. J Endocrinol. 2004; 181(3): 419-27[PubMed]
  • 34. Adams V, Nehrhoff B, Spate U, Linke A, Schulze PC, Baur A, et al. Induction of iNOS expression in skeletal muscle by IL-1beta and NFkappaB activation: an in vitro and in vivo study. Cardiovasc Res. 2002; 54(1): 95-104[PubMed]
  • 35. Gomez-Cabrera MC, Borras C, Pallardo FV, Sastre J, Ji LL, Vina J. Decreasing xanthine oxidase-mediated oxidative stress prevents useful cellular adaptations to exercise in rats. J Physiol. 2005; 567: 113-20[DOI][PubMed]
  • 36. Gilad E, Wong HR, Zingarelli B, Virag L, O'Connor M, Salzman AL, et al. Melatonin inhibits expression of the inducible isoform of nitric oxide synthase in murine macrophages: role of inhibition of NFkappaB activation. FASEB J. 1998; 12(9): 685-93[PubMed]
  • 37. Rabelo SB, Villaverde AB, Nicolau R, Salgado MC, Melo Mda S, Pacheco MT. Comparison between wound healing in induced diabetic and nondiabetic rats after low-level laser therapy. Photomed Laser Surg. 2006; 24(4): 474-9[DOI][PubMed]
  • 38. Albertini R, Aimbire FS, Correa FI, Ribeiro W, Cogo JC, Antunes E, et al. Effects of different protocol doses of low power gallium-aluminum-arsenate (Ga-Al-As) laser radiation (650 nm) on carrageenan induced rat paw ooedema. J Photochem Photobiol B. 2004; 74(2-3): 101-7[DOI][PubMed]
  • 39. Ferreira DM, Zangaro RA, Villaverde AB, Cury Y, Frigo L, Picolo G, et al. Analgesic effect of He-Ne (632.8 nm) low-level laser therapy on acute inflammatory pain. Photomed Laser Surg. 2005; 23(2): 177-81[DOI][PubMed]
  • 40. Albertini R, Villaverde AB, Aimbire F, Salgado MA, Bjordal JM, Alves LP, et al. Anti-inflammatory effects of low-level laser therapy (LLLT) with two different red wavelengths (660 nm and 684 nm) in carrageenan-induced rat paw edema. J Photochem Photobiol B. 2007; 89(1): 50-5[DOI][PubMed]
  • 41. Moriyama Y, Moriyama EH, Blackmore K, Akens MK, Lilge L. In vivo study of the inflammatory modulating effects of low-level laser therapy on iNOS expression using bioluminescence imaging. Photochem Photobiol. 2005; 81(6): 1351-5[DOI][PubMed]
  • 42. Rizzi CF, Mauriz JL, Freitas Correa DS, Moreira AJ, Zettler CG, Filippin LI, et al. Effects of low-level laser therapy (LLLT) on the nuclear factor (NF)-kappaB signaling pathway in traumatized muscle. Lasers Surg Med. 2006; 38(7): 704-13[DOI][PubMed]
  • 43. Gavish L, Asher Y, Becker Y, Kleinman Y. Low level laser irradiation stimulates mitochondrial membrane potential and disperses subnuclear promyelocytic leukemia protein. Lasers Surg Med. 2004; 35(5): 369-76[DOI][PubMed]
  • 44. Gavish L, Perez L, Gertz SD. Low-level laser irradiation modulates matrix metalloproteinase activity and gene expression in porcine aortic smooth muscle cells. Lasers Surg Med. 2006; 38(8): 779-86[DOI][PubMed]
  • 45. Aimbire F, Albertini R, Pacheco MT, Castro-Faria-Neto HC, Leonardo PS, Iversen VV, et al. Low-level laser therapy induces dose-dependent reduction of TNFalpha levels in acute inflammation. Photomed Laser Surg. 2006; 24(1): 33-7[DOI][PubMed]
  • 46. Sakurai Y, Yamaguchi M, Abiko Y. Inhibitory effect of low-level laser irradiation on LPS-stimulated prostaglandin E2 production and cyclooxygenase-2 in human gingival fibroblasts. Eur J Oral Sci. 2000; 108(1): 29-34[PubMed]
  • 47. Brondon P, Stadler I, Lanzafame RJ. A study of the effects of phototherapy dose interval on photobiomodulation of cell cultures. Lasers Surg Med. 2005; 36(5): 409-13[DOI][PubMed]
  • 48. Storz P. Mitochondrial ROS--radical detoxification, mediated by protein kinase D. Trends Cell Biol. 2007; 17(1): 13-8[DOI][PubMed]
  • 49. Low level laser therapy for traumatic brain injury. Mechanisms for Low-Light Therapy V. Proc SPIE.
  • 50. Amat A, Rigau J, Waynant RW, Ilev IK, Anders JJ. The electric field induced by light can explain cellular responses to electromagnetic energy: a hypothesis of mechanism. J Photochem Photobiol B. 2006; 82(2): 152-60[DOI][PubMed]
  • 51. Vladimirov Y. Efferent medicine. 1994; : 51-66
  • 52. Manteifel VM, Karu TI. [Structure of mitochondria and activity of their respiratory chain in subsequent generations of yeast cells exposed to He-Ne laser light]. Izv Akad Nauk Ser Biol. 2005; (6): 672-83[PubMed]
  • 53. Hrnjak M, Kuljic-Kapulica N, Budisin A, Giser A. Stimulatory effect of low-power density He-Ne laser radiation on human fibroblasts in vitro. Vojnosanit Pregl. 1995; 52(6): 539-46[PubMed]
  • 54. Romm AR, Sherstnev MP, Volkov VV, Vladimirov Iu A. [Action of laser radiation on the peroxide chemiluminescence of wound exudate]. Biull Eksp Biol Med. 1986; 102(10): 426-8[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments